The extraordinary world of vegetables
Vegetables, something ordinary for the common person. But have you ever wondered what processes at the genetic level make them grow, making it possible for us to eat them in various forms (e.g. frozen, cooked)
Dr. Pablo Cavagnaro’s team conduct a research project at the University of Agriculture in Krakow, in which they are trying to identify the genes responsible for transitions in plants from the vegetative stage to the reproductive stage, known to all of us as flowering.
The project focuses specifically on vernalization which is the process by which plants use an extended cold period, such as winter, to get ready to flower. The researchers decided to concentrate on the carrot because it is one of the ten most economically important and consumed vegetables worldwide.
In order to produce flowers and seeds, many vegetable plants – including the carrot – first need to overcome their juvenile stage and reach “adulthood”, which happens when the plant reaches a certain vegetative growth threshold (e.g., in carrots the juvenile stage is considered over when the plant has 8-12 leaves and a root diameter of ~ 4-8 mm)Then they need to accumulate a certain number of hours of cold to shift from the vegetative to the reproductive stage. These physiological and biochemical changes take place in the meristem of the plant which is a type of tissue composed of f cells that can divide and grow into all the other tissues and organs in plants.
After the necessary changes are produced in the meristem, the plant is ready to flower, which usually happens after the winter is over. The amount of cold that a given species, or a variety within a species, needs to be exposed to in order to start flowering is called “vernalization requirement” (VR) and it is genetically determined – a given plant variant has a gene, or a set of genes, that condition the amount of cold it requires for flowering. . Previous studies have already helped to narrow down the possible chromosome region where these genes might be located, but the actual genes controlling carrot vernalization requirements have not yet been identified. Dr Cavagnaro’s project is bringing us closer to their discovery!
To accomplish this, the researchers are comparing three early flowering and three late flowering carrots (that is six carrot cultivars with contrasting VR) ) and looking at how their complete set of genes (the “transcriptome”) behaves in vernalized and not vernalized plants. This helps them identify genes that are expressed at different rates in both groups and are related to flowering or vernalization processes. The fact that research is conducted in Poland allows the project to combine different approaches, different expertise, different equipment from the Polish host institution and Argentinian universities, where the Principal Investigator conducted his earlier research.
If we understand more about how vernalization requirements are regulated, we would be able to develop molecular breeding strategies for future crops.
Principal Investigator: prof. dr Pablo Federico Cavagnaro
Project Title: Understanding the genetic basis of carrot vernalization
Project’s website: www.projekty.urk.edu.pl/index/site/8791
